Journal:

Article Title: Helicobacter pylori FlgR Is an Enhancer-Independent Activator of ? 54 -RNA Polymerase Holoenzyme

doi: 10.1128/JB.186.14.4535-4542.2004

Figure Lengend Snippet: Immunoblot of cell extracts of wild-type H. pylori and flgR and flgS mutant strains. Each lane contained cell extracts (50 μg of protein) from one of the H. pylori strains. The blot was probed with antiserum directed against the H. pylori flagellum. Lane 1, wild-type H. pylori ATCC 43504; lane 2, MGD1 (flgR:aphA3 mutant strain); lane 3, MGD2 (flgS:aphA3 mutant strain). FlgE, FlaB, and FlaA bands are indicated.

Article Snippet: This plasmid was introduced into H. pylori strain ATCC 43504 by electroporation, and transformants were selected on TSA containing chloramphenicol.

Techniques: Western Blot, Mutagenesis

Journal:

Article Title: Helicobacter pylori FlgR Is an Enhancer-Independent Activator of ? 54 -RNA Polymerase Holoenzyme

doi: 10.1128/JB.186.14.4535-4542.2004

Figure Lengend Snippet: Expression of flaB ′-′ xylE , flaA ′-′ xylE , and flgI ′-′ xylE reporter genes in various H. pylori strains

Article Snippet: This plasmid was introduced into H. pylori strain ATCC 43504 by electroporation, and transformants were selected on TSA containing chloramphenicol.

Techniques: Expressing, Activity Assay

Journal:

Article Title: Rapid-Cycle PCR and Fluorimetry for Detection of Mycobacteria

doi: 10.1128/JCM.40.9.3364-3373.2002

Figure Lengend Snippet: Profile of 16S rRNA gene amplification and probe hybridization

Article Snippet: M. kansasii (DSMZ 44162) , + , 61.5 , 50 , 48.

Techniques: Amplification

Journal: Antibiotics

Article Title: Synthesis and Evaluation of the Antibacterial Activities of 13-Substituted Berberine Derivatives

doi: 10.3390/antibiotics9070381

Figure Lengend Snippet: Concentration and time-dependent effect of berberine derivatives on the bacterial membrane integrity. The dose- and time-dependent effects of compounds 16 and 25 on bacterial membrane integrity were studied using B. subtilis ( A – C ), H. pylori ( D – F ), S. aureus ( G – I ) or V. alginolyticus ( J – L ). Graphs ( A , D , G , J ) show the dose-dependent effect of 60 min incubation at 37 °C with increasing concentrations of derivatives. Graphs ( B , E , H , K ) show the time-dependent effect at 37 °C of derivative at 4-times their MIC. Graphs ( C , F , I , L ) show the time-dependent effect at 37 °C of derivative at 100 µM. In all cases, results were expressed as percentage of membrane permeabilization using CTAB at 300 µM as positive control giving 100% permeabilization. Results corresponding to means ± SD ( n = 3) were fitted using the Graph Pad Prism software.

Article Snippet: Environmental and pathogenic Gram-negative bacterial strains used were: Acinetobacter baumannii (CIP 110431), Citrobacter farmeri (ATCC 51633), Citrobacter rodentium (ATCC 51116), Escherichia coli (ATCC 8739), Helicobacter pylori (ATCC 43504), Klebsiella pneumoniae (DSMZ 26371), Pseudomonas aeruginosa (ATCC 9027), fluoroquinolone- and carbapenem-resistant Pseudomonas aeruginosa (CIP 107398), Salmonella enterica (CIP 80.39), Shigella flexneri (ATCC 12022), Vibrio alginolyticus (DSM 2171) and Vibrio diabolicus (from Dr Aurélie Tasiemski, Institut Pasteur Lille).

Techniques: Concentration Assay, Membrane, Incubation, Positive Control, Software